Intestinal protozoa Molecular Diagnostics
This scheme consists of three distributions, each comprising three unpreserved stool samples. Analysis by a Nucleic Acid Amplification Technique (NAAT, such asPCR) is requested for the detection of protozoa. The presence or absence of the following intestinal protozoa is validated: Blastocystis spp. Cryptosporidium spp., Dientamoeba fragilis, Entamoeba dispar, Entamoeba histolytica and Giardia lamblia. For the resultswith regard to the presence or absence of Blastocystis spp, no performance score is awarded because this target is validated by a too small number of expert laboratories. Therefore, Blastocystis spp is an educational target. Finally, each distribution is finalizedwith a summarizing discussion in which all findings of the participants are presented.
Contactperson
prof. dr. J.J. van Hellemond (Coördinator)Frequency
3 rounds of 3 samplesNumber of participants
41Price
Participation
€ 274.03
Additional cluster
€ 120.04
Details
This scheme is provided in Dutch and English. International participants should be aware that this scheme use unpreserved samples, so long-distance transport isnot encouraged. Subscription costs do not include shipment by courier in case the participant is based outside the European Union.
For more information, please contact the scheme coordinator: Prof.dr. J.J. van Hellemond e-mail: office@skml.nl.
This scheme falls within the scope of accreditation of the SKML according to ISO/IEC 17043:2010 with registration number R023 at the Dutch Accreditation Council.
Supported analytes
Blastocystis spp. PCR |
Cryptosporidium spp. PCR |
Dientamoeba fragilis PCR |
Entamoeba dispar PCR |
Entamoeba histolytica PCR |
Giardia lamblia PCR |
Methodelijst Darmprotozoa Moleculaire Diagnostiek
Blastocystis spp. PCR | Consensus methodegroep |
---|---|
BD Max Entric Parasite Panel | Overall |
Biofire, Gastrointestinal panel | Overall |
Commercial kit (explain in comments) | Overall |
Congen SureFast MFP4 | Overall |
Lab Dev Test (LDT) ITS | Overall |
Lab Dev Test (LDT) other target | Overall |
Lab Dev Test (LDT) SSU-rRNA | Overall |
Seegene GI-Parasite assay | Overall |
Cryptosporidium spp. PCR | Consensus methodegroep |
---|---|
BD Max Entric Parasite Panel | Overall |
Biofire, Gastrointestinal panel | Overall |
Commercial kit (explain in comments) | Overall |
Congen SureFast MFP4 | Overall |
Lab Dev Test (LDT) 18S rRNA gene | Overall |
Lab Dev Test (LDT) 452 bp specific fragment | Overall |
Lab Dev Test (LDT) DNA-j like protein | Overall |
Lab Dev Test (LDT) other target | Overall |
Lab Dev Test (LDT) SSU-rRNA | Overall |
Seegene GI-Parasite assay | Overall |
Dientamoeba fragilis PCR | Consensus methodegroep |
---|---|
BD Max Entric Parasite Panel | Overall |
Biofire, Gastrointestinal panel | Overall |
Commercial kit (explain in comments) | Overall |
Congen SureFast MFP4 | Overall |
Lab Dev Test (LDT) 5.8S rRNA gene | Overall |
Lab Dev Test (LDT) 5S spacer fragement | Overall |
Lab Dev Test (LDT) other target | Overall |
Lab Dev Test (LDT) SSU-rRNA | Overall |
Seegene GI-Parasite assay | Overall |
Entamoeba dispar PCR | Consensus methodegroep |
---|---|
BD Max Entric Parasite Panel | Overall |
Biofire, Gastrointestinal panel | Overall |
Commercial kit (explain in comments) | Overall |
Congen SureFast MFP4 | Overall |
Lab Dev Test (LDT) other target | Overall |
Lab Dev Test (LDT) SSU-rRNA | Overall |
Entamoeba histolytica PCR | Consensus methodegroep |
---|---|
BD Max Entric Parasite Panel | Overall |
Biofire, Gastrointestinal panel | Overall |
Commercial kit (explain in comments) | Overall |
Congen SureFast MFP4 | Overall |
Lab Dev Test (LDT) 18S rRNA gene | Overall |
Lab Dev Test (LDT) other target | Overall |
Lab Dev Test (LDT) SSU-rRNA | Overall |
Seegene GI-Parasite assay | Overall |
Giardia lamblia PCR | Consensus methodegroep |
---|---|
BD Max Entric Parasite Panel | Overall |
Biofire, Gastrointestinal panel | Overall |
Commercial kit (explain in comments) | Overall |
Congen SureFast MFP4 | Overall |
Lab Dev Test (LDT) 18S rRNA gene | Overall |
Lab Dev Test (LDT) other target | Overall |
Lab Dev Test (LDT) SSU-rRNA | Overall |
Seegene GI-Parasite assay | Overall |
Analyzer
Analyzer | Manufacturer |
---|---|
BD-Max | Becton Dickinson |
Biofire - spotfire | bioMérieux |
Biomerieux (easyMag) | bioMérieux |
Other equipment | Overigen |
Qiagen (QIA symphony) | Qiagen |
Qiagen (Stool kit mini) | Qiagen |
Roche (Magnapure) | Roche Diagnostics |
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