Intestinal protozoa Molecular Diagnostics

This scheme consists of three distributions, each comprising three unpreserved stool samples. Analysis by a Nucleic Acid Amplification Technique (NAAT, such asPCR) is requested for the detection of protozoa. The presence or absence of the following intestinal protozoa is validated: Blastocystis spp. Cryptosporidium spp., Dientamoeba fragilis, Entamoeba dispar, Entamoeba histolytica and Giardia lamblia. For the resultswith regard to the presence or absence of Blastocystis spp, no performance score is awarded because this target is validated by a too small number of expert laboratories. Therefore, Blastocystis spp is an educational target. Finally, each distribution is finalizedwith a summarizing discussion in which all findings of the participants are presented.

Frequency

3 rounds of 3 samples

Number of participants

41

Price

Participation
€ 274.03
Additional cluster
€ 120.04

Details

This scheme is provided in Dutch and English. International participants should be aware that this scheme use unpreserved samples, so long-distance transport isnot encouraged. Subscription costs do not include shipment by courier in case the participant is based outside the European Union.
For more information, please contact the scheme coordinator: Prof.dr. J.J. van Hellemond e-mail: office@skml.nl.

This scheme falls within the scope of accreditation of the SKML according to ISO/IEC 17043:2010 with registration number R023 at the Dutch Accreditation Council.

Supported analytes

Blastocystis spp. PCR
Cryptosporidium spp. PCR
Dientamoeba fragilis PCR
Entamoeba dispar PCR
Entamoeba histolytica PCR
Giardia lamblia PCR

Methodelijst Darmprotozoa Moleculaire Diagnostiek

Blastocystis spp. PCRConsensus methodegroep
BD Max Entric Parasite Panel Overall
Biofire, Gastrointestinal panel Overall
Commercial kit (explain in comments) Overall
Congen SureFast MFP4 Overall
Lab Dev Test (LDT) ITS Overall
Lab Dev Test (LDT) other target Overall
Lab Dev Test (LDT) SSU-rRNA Overall
Seegene GI-Parasite assay Overall
Cryptosporidium spp. PCRConsensus methodegroep
BD Max Entric Parasite Panel Overall
Biofire, Gastrointestinal panel Overall
Commercial kit (explain in comments) Overall
Congen SureFast MFP4 Overall
Lab Dev Test (LDT) 18S rRNA gene Overall
Lab Dev Test (LDT) 452 bp specific fragment Overall
Lab Dev Test (LDT) DNA-j like protein Overall
Lab Dev Test (LDT) other target Overall
Lab Dev Test (LDT) SSU-rRNA Overall
Seegene GI-Parasite assay Overall
Dientamoeba fragilis PCRConsensus methodegroep
BD Max Entric Parasite Panel Overall
Biofire, Gastrointestinal panel Overall
Commercial kit (explain in comments) Overall
Congen SureFast MFP4 Overall
Lab Dev Test (LDT) 5.8S rRNA gene Overall
Lab Dev Test (LDT) 5S spacer fragement Overall
Lab Dev Test (LDT) other target Overall
Lab Dev Test (LDT) SSU-rRNA Overall
Seegene GI-Parasite assay Overall
Entamoeba dispar PCRConsensus methodegroep
BD Max Entric Parasite Panel Overall
Biofire, Gastrointestinal panel Overall
Commercial kit (explain in comments) Overall
Congen SureFast MFP4 Overall
Lab Dev Test (LDT) other target Overall
Lab Dev Test (LDT) SSU-rRNA Overall
Entamoeba histolytica PCRConsensus methodegroep
BD Max Entric Parasite Panel Overall
Biofire, Gastrointestinal panel Overall
Commercial kit (explain in comments) Overall
Congen SureFast MFP4 Overall
Lab Dev Test (LDT) 18S rRNA gene Overall
Lab Dev Test (LDT) other target Overall
Lab Dev Test (LDT) SSU-rRNA Overall
Seegene GI-Parasite assay Overall
Giardia lamblia PCRConsensus methodegroep
BD Max Entric Parasite Panel Overall
Biofire, Gastrointestinal panel Overall
Commercial kit (explain in comments) Overall
Congen SureFast MFP4 Overall
Lab Dev Test (LDT) 18S rRNA gene Overall
Lab Dev Test (LDT) other target Overall
Lab Dev Test (LDT) SSU-rRNA Overall
Seegene GI-Parasite assay Overall

Analyzer

AnalyzerManufacturer
BD-Max Becton Dickinson
Biofire - spotfire bioMérieux
Biomerieux (easyMag) bioMérieux
Other equipment Overigen
Qiagen (QIA symphony) Qiagen
Qiagen (Stool kit mini) Qiagen
Roche (Magnapure) Roche Diagnostics

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