Lymfocyten subsets BAL

Monsters uit broncho-alveolair lavages worden gestabiliseerd en rondgestuurd met klinische informatie en vraagstelling. Gevraagd onderzoek: celconcentratie, differentiatie leukocyten, en immuunfenotypering lymfocyten (CD3+, CD4+, CD8+ T lymfocten; CD19+ B lymfocyten; NK lymfocyten en coëxpressie van CD103 door T-cel subsets). De deelnemers dienen een conclusie en advies aan de behandelend arts te formuleren.

Een link naar de protocollen zijn te vinden op de sectiepagina IMCD.

Contactpersoon

R.F.M. Broekkamp (Coördinator)

Frequentie

2 rondes van 1 monsters

Aantal deelnemers

Prijzen

Deelname

€ 367,65

Extra cluster

€ 78,29

Deelnemen

Details

Elk jaar worden de resultaten plenair en anoniem besproken tijdens het najaars NVC-SKML congres.

Verwerking

Deze rondzending wordt elektronisch verwerkt en gerapporteerd (QBase).

Ondersteunde bepalingen

% pos macrofagen
CD103
CD103,CD4/CD4
CD19+
CD3+
CD3+CD4+
CD3+CD8+
CD3-CD16+CD56+
Eosinofiele granulocyten
Golde score
Lymfo's op flow
Lymfocyten
Macrofagen
Mestcellen
Neutrofiele granulocyten
Plasmacellen

Methodelijst Lymfocyten subsets BAL

% pos macrofagen	Consensus methodegroep
Pruisische kleuring	Overall

CD103	Consensus methodegroep
A-700	Overall
A750	Overall
APC	Overall
ECD	Overall
FITC	Overall
KO	Overall
overig	Overall
PB	Overall
PC5	Overall
PC5.5	Overall
PC7	Overall
PE	Overall
PerCp	Overall

CD103,CD4/CD4	Consensus methodegroep
A-700	Overall
A750	Overall
APC	Overall
ECD	Overall
FITC	Overall
KO	Overall
overig	Overall
PB	Overall
PC5	Overall
PC5.5	Overall
PC7	Overall
PE	Overall
PerCp	Overall

CD19+	Consensus methodegroep
A-700	Overall
A750	Overall
APC	Overall
ECD	Overall
FITC	Overall
KO	Overall
overig	Overall
PB	Overall
PC5	Overall
PC5.5	Overall
PC7	Overall
PE	Overall
PerCp	Overall

CD3+	Consensus methodegroep
A-700	Overall
A750	Overall
APC	Overall
ECD	Overall
FITC	Overall
KO	Overall
overig	Overall
PB	Overall
PC5	Overall
PC5.5	Overall
PC7	Overall
PE	Overall
PerCp	Overall

CD3+CD4+	Consensus methodegroep
A-700	Overall
A750	Overall
APC	Overall
ECD	Overall
FITC	Overall
KO	Overall
overig	Overall
PB	Overall
PC5	Overall
PC5.5	Overall
PC7	Overall
PE	Overall
PerCp	Overall

CD3+CD8+	Consensus methodegroep
A-700	Overall
A750	Overall
APC	Overall
ECD	Overall
FITC	Overall
KO	Overall
overig	Overall
PB	Overall
PC5	Overall
PC5.5	Overall
PC7	Overall
PE	Overall
PerCp	Overall

CD3-CD16+CD56+	Consensus methodegroep
A-700	Overall
A750	Overall
APC	Overall
ECD	Overall
FITC	Overall
KO	Overall
overig	Overall
PB	Overall
PC5	Overall
PC5.5	Overall
PC7	Overall
PE	Overall
PerCp	Overall

Eosinofiele granulocyten	Consensus methodegroep
MGG kleuring	Overall
overig	Overall

Golde score	Consensus methodegroep
Pruisische kleuring	Overall

Lymfo's op flow	Consensus methodegroep
CD45/SSC gating	Overall
FSC/SSC gating	Overall
overig	Overall

Lymfocyten	Consensus methodegroep
MGG kleuring	Overall
overig	Overall

Macrofagen	Consensus methodegroep
MGG kleuring	Overall
overig	Overall

Mestcellen	Consensus methodegroep
MGG kleuring	Overall
overig	Overall

Neutrofiele granulocyten	Consensus methodegroep
MGG kleuring	Overall
overig	Overall

Plasmacellen	Consensus methodegroep
MGG kleuring	Overall
overig	Overall

Apparaten

Apparaat	Fabrikant
Cell-Dyn 1200	Abbott
Cell-Dyn 1300	Abbott
Cell-Dyn 1700	Abbott
Cell-Dyn 1800	Abbott
Cell-Dyn 3200	Abbott
Cell-Dyn 3500	Abbott
Cell-Dyn 3700	Abbott
Cell-Dyn 4000	Abbott
Cell-Dyn Emerald	Abbott
i-STAT	Abbott
Sapphire	Abbott
ABX Argos	ABX
ABX Micros60 (Bayer 60)	ABX
ABX Pentra 120	ABX
Beckman-Coulter DxH 800 Coulter Cellular Analysis	Beckman-Coulter
Beckman-Coulter flowcytometer Navios	Beckman-Coulter
Beckman-Coulter Gen-S	Beckman-Coulter
Beckman-Coulter LH750	Beckman-Coulter
Beckman-Coulter overigen	Beckman-Coulter
HmX	Beckman-Coulter
STKS / MAXM	Beckman-Coulter
Becton Dickinson	Becton Dickinson
FACS Canto	Becton Dickinson
Variant Hemoglobin Testing System	Bio-Rad
TOSOH G7	Goffin Meyvis
TOSOH G8	Goffin Meyvis
Hemocue	Hemocue
Medonic	Medonic
Menarini HA-8160	Menarini Diagnostics
NOVA CO-Oximeter	Nova Biomedical
Ortho	Ortho Clinical Diagnostics
Manueel	Overigen
Microscoop	Overigen
Overigen / Not specified	Overigen
ABL-800 serie	Radiometer Nederland
NPT7	Radiometer Nederland
Roche OMNI	Roche Diagnostics
Advia 120/2120	Siemens Healthcare Diagnostics
Advia 60	Siemens Healthcare Diagnostics
Bayer Advia 2120	Siemens Healthcare Diagnostics
Bayer H1	Siemens Healthcare Diagnostics
Bayer H1 junior	Siemens Healthcare Diagnostics
Bayer H2	Siemens Healthcare Diagnostics
Bayer H3	Siemens Healthcare Diagnostics
Bayer Rapidlab 840/845	Siemens Healthcare Diagnostics
Bayer Rapidlab 850/855	Siemens Healthcare Diagnostics
Bayer Rapidlab 860/865	Siemens Healthcare Diagnostics
Rapidlab 1200 serie	Siemens Healthcare Diagnostics
Rapidlab 248/348	Siemens Healthcare Diagnostics
Rapidlab 400 serie	Siemens Healthcare Diagnostics
Rapidlab 800 serie	Siemens Healthcare Diagnostics
Sysmex CC-serie	Sysmex
Sysmex E+M+K-serie	Sysmex
Sysmex NE1500	Sysmex
Sysmex NE4000	Sysmex
Sysmex NE5000	Sysmex
Sysmex NE8000	Sysmex
Sysmex overige typen	Sysmex
Sysmex POCH 100i	Sysmex
Sysmex SE9000	Sysmex
Sysmex SE9000	Sysmex
Sysmex XE5000	Sysmex
Sysmex XS1000i	Sysmex
Sysmex XT1800i	Sysmex
Sysmex XT2000i	Sysmex
Sysmex XT4000i	Sysmex

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