PNH diagnostiek

Per jaar worden 3 verse bloedmonsters van patiënten die bekend zijn met Paroxysmale Nachtelijke Hemoglobinurie rondgestuurd met vraagstelling: hoe groot is de PNH kloon in de granulocyten, monocyten en de erythrocyten. Gevraagd onderzoek: immuunfenotypering van granulocyten, monocyten en de erythrocyten met behulp van eigen panel aan MoAbs die binden aan antigenen die door middel van een GPI-anker met het membraan verbonden zijn. De deelnemers dienen het percentage PNH-kloon in granulocyten, monocyten en de erythrocyten weer te geven als ook waarop deze conclusie gebaseerd is.

Contactpersoon

dr. W. Hobo (Coördinator)

Frequentie

3 rondes van 1 monsters

Aantal deelnemers

Prijzen

Deelname

€ 277,10

Deelnemen

Details

Eenmaal per jaar worden de resultaten van de rondzendingen plenair en anoniem besproken. GPI-verankerde antigenen op granulocyten, monocyten en de erythrocyten.

Verwerking

Deze rondzending wordt elektronisch verwerkt en gerapporteerd (QBase).

Ondersteunde bepalingen

CD14 PNH kloon (monocyten)
CD157 PNH kloon (monocyten)
CD157 PNH kloon (neutrofielen)
CD16 PNH kloon (neutrofielen)
CD24 PNH kloon (neutrofielen)
CD55 PNH kloon (monocyten)
CD55 PNH kloon (neutrofielen)
CD55 PNH kloon totaal
CD55 Type II cellen
CD55 Type III cellen
CD58 PNH kloon totaal
CD58 Type II cellen
CD58 Type III cellen
CD59 PNH kloon (monocyten)
CD59 PNH kloon (neutrofielen)
CD59 PNH kloon totaal
CD59 Type II cellen
CD59 Type III cellen
Conclusie PNH
Conclusie PNH kloon (type II + III)
FLAER PNH kloon (monocyten)
FLAER PNH kloon (neutrofielen)
Samenstelling bloed

Methodelijst PNH diagnostiek

CD14 PNH kloon (monocyten)	Consensus methodegroep
Alexa 488	Overall
Alexa 700	Overall
APC	Overall
APC A750	Overall
APC-Cy7	Overall
APC-H7	Overall
ECD	Overall
FITC	Overall
PB (Pacific Blue)	Overall
PE	Overall
PE-Cy5	Overall
PE-Cy7	Overall
PerCp	Overall

CD157 PNH kloon (monocyten)	Consensus methodegroep
Alexa 488	Overall
APC	Overall
APC-Cy7	Overall
ECD	Overall
FITC	Overall
PE	Overall
PE-Cy5	Overall
PE-Cy7	Overall
PerCp	Overall

CD157 PNH kloon (neutrofielen)	Consensus methodegroep
Alexa 488	Overall
APC	Overall
APC-Cy7	Overall
ECD	Overall
FITC	Overall
PE	Overall
PE-Cy5	Overall
PE-Cy7	Overall
PerCp	Overall

CD16 PNH kloon (neutrofielen)	Consensus methodegroep
Alexa 488	Overall
APC	Overall
APC-Cy7	Overall
ECD	Overall
FITC	Overall
PE	Overall
PE-Cy5	Overall
PE-Cy7	Overall
PerCp	Overall

CD24 PNH kloon (neutrofielen)	Consensus methodegroep
Alexa 488	Overall
APC	Overall
APC-Cy7	Overall
ECD	Overall
FITC	Overall
PE	Overall
PE-Cy5	Overall
PE-Cy7	Overall
PerCp	Overall

CD55 PNH kloon (monocyten)	Consensus methodegroep
Alexa 488	Overall
APC	Overall
APC-Cy7	Overall
ECD	Overall
FITC	Overall
PE	Overall
PE-Cy5	Overall
PE-Cy7	Overall
PerCp	Overall

CD55 PNH kloon (neutrofielen)	Consensus methodegroep
Alexa 488	Overall
APC	Overall
APC-Cy7	Overall
ECD	Overall
FITC	Overall
PE	Overall
PE-Cy5	Overall
PE-Cy7	Overall
PerCp	Overall

CD55 PNH kloon totaal	Consensus methodegroep
Alexa 488	Overall
APC	Overall
FITC	Overall
PE	Overall
PerCp	Overall

CD55 Type II cellen	Consensus methodegroep
Alexa 488	Overall
APC	Overall
FITC	Overall
PE	Overall
PerCp	Overall

CD55 Type III cellen	Consensus methodegroep
Alexa 488	Overall
APC	Overall
FITC	Overall
PE	Overall
PerCp	Overall

CD58 PNH kloon totaal	Consensus methodegroep
Alexa 488	Overall
APC	Overall
FITC	Overall
PE	Overall
PerCp	Overall

CD58 Type II cellen	Consensus methodegroep
Alexa 488	Overall
APC	Overall
FITC	Overall
PE	Overall
PerCp	Overall

CD58 Type III cellen	Consensus methodegroep
Alexa 488	Overall
APC	Overall
FITC	Overall
PE	Overall
PerCp	Overall

CD59 PNH kloon (monocyten)	Consensus methodegroep
Alexa 488	Overall
APC	Overall
APC-Cy7	Overall
ECD	Overall
FITC	Overall
PE	Overall
PE-Cy5	Overall
PE-Cy7	Overall
PerCp	Overall

CD59 PNH kloon (neutrofielen)	Consensus methodegroep
Alexa 488	Overall
APC	Overall
APC-Cy7	Overall
ECD	Overall
FITC	Overall
PE	Overall
PE-Cy5	Overall
PE-Cy7	Overall
PerCp	Overall

CD59 PNH kloon totaal	Consensus methodegroep
Alexa 488	Overall
APC	Overall
FITC	Overall
PE	Overall
PerCp	Overall

CD59 Type II cellen	Consensus methodegroep
Alexa 488	Overall
APC	Overall
FITC	Overall
PE	Overall
PerCp	Overall

CD59 Type III cellen	Consensus methodegroep
Alexa 488	Overall
APC	Overall
FITC	Overall
PE	Overall
PerCp	Overall

FLAER PNH kloon (monocyten)	Consensus methodegroep
Alexa 488	Overall
APC	Overall
APC-Cy7	Overall
ECD	Overall
FITC	Overall
PE	Overall
PE-Cy5	Overall
PE-Cy7	Overall
PerCp	Overall

FLAER PNH kloon (neutrofielen)	Consensus methodegroep
Alexa 488	Overall
APC	Overall
APC-Cy7	Overall
ECD	Overall
FITC	Overall
PE	Overall
PE-Cy5	Overall
PE-Cy7	Overall
PerCp	Overall

Samenstelling bloed	Consensus methodegroep
"Andere" (specificeer bij opmerking)	Overall
Lyse Stain Wash	Overall
Stain Lyse Wash	Overall

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